OTHERTOOLSANDRESOURCES  

CHEMICALSTRUCTURE

 3,5-difluoro-4-hydroxybenzylideneimidazolinone-2-oxime(DHFO)

MOLECULARFORMULA:

C₁₂H₈F₂N₃O₃

MOLECULARWEIGHT:

281.2

CASNAME/NUMBER:

(E)-4-((Z)-3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehydeoxime / 

FLUORESCENCESPECTRA

A.AbsorptionandfluorescenceemissionspectraofCorn^TM/DFHOinpH7.4buffer.  B. AbsorptionandfluorescenceemissionspectraofBroccoli^TM/DFHOinpH7.4buffer.  

PRESENTATION: Lyophilizeddye

QUALITYASSURANCE: Productsareanalyzedby ¹HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucerna^TM productswillbehaveinitscustomers"systems.ResearchersusingLucerna^TM productsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

DFHOIMAGES 

A. Live-cellimagingofHEK293TcellsexpressingU6promoterdrivenCorn^TM inthepresenceof10µMDFHO.ImagesareHoechst-stainednuclei(blue)andCorn^TM fluorescence(yellow),individuallyoroverlayed. ImageswereacquiredusingYFPandDAPIfiltersets.  B. Live-cellimagingofE.colicellsexpressingtRNApromoterdrivenCorn^TM inthepresenceof10µMDFHO.

REFERENCEFORTHEPRODUCT:

SongW,FilonovGS,KimH,HirschM,LiX,MoonJD,JaffreySR.2017. ImagingRNApolymeraseIIItranscriptionusingaphotostableRNA-fluorophorecomplex. NatChemBiol.

THISPRODUCTWASCITEDIN: 

LICENSING: 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense.