OTHERTOOLSANDRESOURCES 

CHEMICALSTRUCTURE

3,5-difluoro-4-hydroxybenzylideneimidazolinone(DFHBI) 

MOLECULARFORMULA:

C₁₂H₁₀F₂N₂O₂

MOLECULARWEIGHT:

252.22

CASNAME/NUMBER:

4H-Imidazol-4-one,5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-,5(Z)- /1241390-29-3

FLUORESCENCESPECTRA

AbsorptionandfluorescenceemissionspectraofDFHBIinpH7.4buffer.

PRESENTATION: Lyophilizeddye

QUALITYASSURANCE: Productsareanalyzedby ¹HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

USAGESTATEMENT:Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucerna^TM productswillbehaveinitscustomers"systems.ResearchersusingLucerna^TMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

DFHBIIMAGES 

HEK293Tcellsweretransfectedwitheither5S-controlaptameror5S-Spinach^TM plasmidsandincubatedwith20µMDFHBI.ImagesarephaseoverlaywithHoechst-stainednuclei(blue)andSpinach^TM fluorescence(green).

Spinach^TM RNArobustlyactivatesthefluorescenceofDFHBI. EachtubecontainedtheindicatedsolutionandwasirrADIatedat465nm.

REFERENCEFORTHEPRODUCT:

RNAmimicsofgreenfluorescentprotein.PaigeJS,WuKY,JaffreySR. Science.2011Jul29;333(6042):642-6.

THISPRODUCTWASCITEDIN: 

RNAsignalamplifiercircuitwithintegratedfluorescenceoutput.AkterFandYokobayashiY.ACSSynthBiol.2015May15.

Design,synthesis,andapplicationofSpinachmolecularbeaconstriggeredbystranddisplacement.BhadraSandEllingtonAD.MethodsEnzymol.2015Jan15.

Live-cellimagingofmammalianRNAswithSpinach2.StrackRLandJaffreySR.MethodsEnzymol.2015Jan6.

MonitoringmRNAandproteinlevelsinbulkandinmodelvesicle-basedartificalcells.vanNiesP,CantonAS,Nourianz,DanelionC.MethodsEnzymol.2015Jan6.

UsingSpinachaptamertocorrelatemRNAandproteinlevelsinEscherichiacoli.PothoulakisGandEllisT.MethodsEnzymol.2015Jan5.

Invitroanalysisofriboswitch-Spinachaptamerfusionsasmetabolite-sensingfluorescentbiosensors.KellenbergerCAandHammondMC.MethodsEnzymol.2014Dec19.

TheSpinachRNAAptamerasaCharacterizationToolforSyntheticBIOLOGy. PothoulakisG,CeroniF,ReeveB,EllisT. ACSSynBiol.2013Sep13.

AsuperfoldingSpinach2revealsthedynamicnatureoftrinucleotiderepeat-containingRNA.StrackRL,DisneyMD,JaffreySR. NatMehtods.2013Dec;10(12):1219-24.

Genepositionmorestronglyinfluencescell-freeproteinexpressionfromoperonsthanT7transcriptionalpromoterstrength.ChizzoliniF,ForlinM,CecchiD,MansySS. ACSSynBiol.2013Dec9.   

LICENSING: 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense.