OTHERTOOLSANDRESOURCES
REFERENCEFORTHEPRODUCT:
Globalanalysisoflysineubiquitinationbyubiquitinremnantimmunoaffinityprofiling. XuG,PaigeJS,JaffreySR.NatureBiotechnology. 2010Aug;28(8)868-73.
THISPRODUCTWASCITEDIN:
A proteome-wide, quantitative survey ofinvivo ubiquitylation sites reveals widespread regulatory roles. WagnerSA, etal. Molecular&CellularProteomics. 2011Sep.
Proteomicanalysesrevealdivergentubiquitylationsitepatternsinmurinetissues.WagnerSA,etal. Molecular&CellularProteomics.2012Jul.
Systems-wideanalysisofubiquitylationdynamicsrevealsakeyroleforPAF15ubiquitylationinDNA-damagebypass.PovlsenLK,etal. NatureCellBIOLOGy. 2012Sep.
| SpeciesReactivity | KeyApplications | Host | Format | AntibodyType |
| Human,mouse,rat,chicken,yeast,cowhorse | WB,IP | Mouse | Purified | MonoclonalAntibody |
QUALITYASSURANCE:EachantibodybatchismeasuredforitsABIlitytobindtoproteinscontainingdiglycyl-lysineandtorecoverdiglycyl-lysine-containingpeptidesfromtrypticdigestsofHEK293cells.
USAGESTATEMENT:Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureofantibodiesandantigens,wecannotpredictorbeheldresponsIBLewithrespecttohowLucernaTMproductswillbehaveinitscustomers"systems.ResearchersusingLucernaTMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication.
LICENSING:CornellUniversityhasfiledausepatentonproteomicmethodsthatusethediglycyl-lysineantibodytoidentifyubiquitinatedproteins.Allcommercial usersshouldcontactCornellUniversityforauselicense.
GX41antibodyrecognizesthediglycyl-lysinemoietyatubiquitinatedsitesaftertrypsindigestion
Specificityofanti-diglycyl-lysine(cloneGX41)isverifiedbywesternblotanalysisofbeta-lactoglobulin,lysozyme,orratbrainlysate,inwhichthelysineswereeitherunmodified(A)ormodifiedwithGly-Gly(B),respectively. Asshownabove,theGX41exhibitsnodetectablereactivitywithproteinsunlessthelysinesaremodifiedwithGly-Gly.
Equimolaramountsofthetwopeptides(GGDRVYIHPFHLandAc-SYSMEHFRWGK*PV-NH2)wereimmunoprecipitatedwithimmobilizedGX41antibodyandanalyzedbyMALDI-TOFMS.K*referstoalysinewithaGly-Glymodification. Asshownabove,theunmodifiedpeptideisabsentfromtheimmunoprecipitate,whilethepeptidecontainingtheGly-Glymodificationisimmunoprecipitated.
LucernaTM旨在通过开发一系列工具包将Spinach技术商业化，使研究人员能够轻松地将两种mRNA与活细胞中的非编码RNA进行成像，从而深入了解这些RNA在细胞生物学和疾病发病机制中的功能。我们的技术目前尚不清楚但可解决的领域包括RNA运输，RNA降解，毒性RNA聚集以及潜在的其他RNA调节机制。我们相信菠菜技术将大大促进RNA的细胞生物学研究，类似于荧光蛋白技术加速细胞对蛋白质的理解。
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