OTHERTOOLSANDRESOURCES  

CHEMICALSTRUCTURE

(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one (DFHBI-1T) 

MOLECULARFORMULA:

C₁₃H₉F₅N₂O₂

MOLECULARWEIGHT:

320.21

CASNAME/NUMBER:

4H-Imidazol-4-one,5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-,(5Z)/1539318-36-0

FLUORESCENCESPECTRA

AbsorptionandfluorescenceemissionspectraofSpinach2^TM/DFHBI-1TinpH7.4buffer.

PRESENTATION: Lyophilizeddye

QUALITYASSURANCE: Productsareanalyzedby¹HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucerna^TM productswillbehaveinitscustomers"systems.ResearchersusingLucerna^TMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

DFHBI-1TIMAGES 

A.Live-cellimagingofaCOS7cellexpressingCGG₆₀-Spinach2^TM inthepresenceofeither20µMDFHBIorDFHBI-1T. Imagesofthesamecellwereacquiredusinga100msecexposurewithaGFPfilterset. B.Quantificationofaverage DFHBI-1Tbrightnessof10cellsexpressingCGG₆₀-Spinach^TM normalizedtothebrightnessofDFHBI.

REFERENCEFORTHEPRODUCT:

SongW,StrackRL,SvensenN,JaffreySR.2014. Plug-and-PlayFluorophoresExtendtheSpectral PropertiesofSpinach.JACS136(4):1198-1201.

THISPRODUCTWASCITEDIN: 

SvensenN,JaffreySR.2016. FluorescentRNAaptamersasatooltostudyRNA-modifyingenzymes. CellChemBiol23(3):415-25.

AwSS,Tang,MX,TeoYN,CohenSM.2016.Aconformation-inducedfluorescencemethodformicroRNAdetection. NucleicAcidsRes44(10):e92.

AlamKK,TawiahKD,LichteMF,Porciani,BurkeDH.2017.AfluorescentsplitaptamerforvisualizingRNA-RNAassemblyinvivo. ACSSynthBiol6(9):1710-1721.

LICENSING: 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense.